We have now extended our FrameStar range to include PCR plates with optically clear flat bottom wells for microscopy applications. The FrameStar 96 Well Skirted Optical Bottom PCR Plates (next to their counterpart within our Individual Access range, 4ti-0970/RA) are currently unique in the market, being suitable for use in both microscopy and PCR.
Optical bottom plates are ideal for applications requiring single cell sorting followed by molecular biology techniques such as (q)PCR and sequencing. The flat bottoms enable excellent stackability, making these plates well suited for small sample volume storage such as compound libraries, with no risk of damaging the seal of the plate below. Additionally, the small well volume enables excellent sample recovery.
The low profile wells of this plate are shorter than "standard" profile wells, which decreases the "dead space" between the heated lid of the thermal cycler and the reagents in the well. This reduces condensation forming on the side wall of the tubes, moderating changes in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20µl.
The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR, and makes it ideal for use with robotic systems.
Parameter: FrameStar 96 Well Skirted Optical Bottom PCR Plate
Plate length: 127.70 ± 0.25mm
Plate width: 85.48 ± 0.25mm
Plate height: 16.10 ± 0.25mm
Well depth: 12 ± 0.10mm
Well diameter: 5.46 ± 0.10mm
Total well capacity: 180µl
Working volume: <0.1ml (100µl)
Distance to center of A1 from top edge: 11.24 ± 0.25mm
Distance to center of A1 from left edge: 14.38 ± 0.25mm
Pitch (distance between A1 and A2): 9.00mm