100um

Falcon® 100 µm cell strainer is a sterile, easy-to-use device for rapidly isolating primary cells to consistently obtain a uniform single-cell suspension from tissues. Applications include stem cells and primary cells. Individually packaged, 50/case

Features

• Strong nylon mesh with 100- micron pores for optimal performance in a variety of applications
• Evenly spaced mesh pores providing consistent and reliable results
• Conveniently accessible in individual packaging
• Extended lip on strainer enables aseptic handling with forceps
• Molded color-coded polypropylene frame with tab enables easy handling and identification
• Fits perfectly into 50 mL Falcon Conical Tube
• Disposable, easy-to-use, inexpensive, maintains sample integrity
• Sterilized by gamma irradiation, noncytotoxic

Application

• Faster, easier alternative to gauze filtration
• Ideal for preparation of flow cytometry samples, including:

• Single cell suspensions of blood cells from marrow, pancreas, thymus, tonsil, and lymph nodes
• Stem cells, tissue-derived cells, and cancer cells
• Preparation of specimens for primary cell cultures and immunogens
• Preparation of freezing stocks
• Filtering agglutinative proteins produced in inactivation serum

Corning® 100µm Cell Strainer is a sterile, easy-to-use device for rapidly isolating primary cells to consistently obtain a uniform single-cell suspension from tissues. Applications include stem cells and primary cells. Individually packaged, 50/case

Features

• Strong nylon mesh with 100- micron pores for optimal performance in a variety of applications
• Evenly spaced mesh pores providing consistent and reliable results
• Conveniently accessible in individual packaging
• Extended lip on strainer enables aseptic handling with forceps
• Molded color-coded polypropylene frame with tab enables easy handling and identification
• Fits all major brands of 50mL Conical Tube
• Disposable, easy-to-use, inexpensive, maintains sample integrity
• Sterilized by gamma irradiation, noncytotoxic

Application

• Faster, easier alternative to gauze filtration
• Ideal for preparation of flow cytometry samples, including:

• Single cell suspensions of blood cells from marrow, pancreas, thymus, tonsil, and lymph nodes
• Stem cells, tissue-derived cells, and cancer cells
• Preparation of specimens for primary cell cultures and immunogens
• Preparation of freezing stocks
• Filtering agglutinative proteins produced in inactivation serum

Cell Strainers of SPL Life Sciences are ideal for obtaining uniform single cell suspension from various sources. Cell strainers are made from nylon with 3 different pore size meshes, showing optimal performances in a variety of applications such as stem cell and primary cell preparation.

▪ Ideal for stem cell and tissue-derived primary cell preparation

▪ Fits into SPL 50 ml conical tubes (Please inquire for other conical tubes)

▪ Non – pyrogenic

▪ Non – cytotoxic

▪ DNase / RNase – free

▪ Human DNA – free

The pluriStrainer® is a sieving device (or cell strainer) for a wide range of laboratory applications where the filtration and the purification of liquids is required. Its unique design features improved ventilation to avoid clogging for a smart sample preparation. pluriStrainer® are stackable to allow direct filtration with different mesh sizes and alsocan be inverted to recover the sieved material. For large sample volumes it can be combined with a funnel. It fits all major brand 50 mL centrifuge (conical) tubes. The combination of the pluriStrainer® and the Connector Ring allow using low pressure which supports the filtration. Furthermore it is possible to block the flow through the sieve and hold back the liquid on top of the mesh. The liquid on top of the mesh can be used for sample preparation and incubation such as tissue dissociation or the treatment of cells with cell lysis reagents (e.g. Buffer RLT, TRIzol®).

• Filter

Attach pluriStrainer® to a sterile 50 mL centrifuge tube. Then, add sample material onto the strainer and filter sample

• Reverse

To obtain the larger fraction, take off the pluriStrainer®, turn it upside down onto another 50 mL tube and flush back the sample from the pluriStrainer®.

• Increase sample load

With Funnel You can add up to 24 mL sample material on top.

• Stack

Stacking of pluriStrainer® with different mesh sizes allows for straining various cell sizes at the same time.

• Flow control

Allows to control the rate of flow by opening or closing the Luer-Lock, e.g. for physical dissociation of primary tissue (brain, spleen etc.).

• Low pressure

If you add a syringe to the Connector Ring, it is possible to force low pressure to support the straining of rough sample material while pulling the piston.

Application

• Obtaining a real single cell suspension after digestion of mammary tissue and organoids
• Enrichment of specific cells using pluriBead technology
• Preparation of a single cell suspension of blood cells from bone marrow, pancreas, thymus, lymph nodes and others
• Dissociation of cells from other primary tissue
• Preparation of real single cell suspension for flow cytometry(FACS™)
• Faster and easier alternative to gauze filtration
• Sieving of complex and viscous liquids (in combination with Connector Ring & Syringe for vacuum)
• Short time incubation of cells / cytokine (in combination with pluriBead and Connector Ring)

Mesh Size: 100 µm

Color: neon yellow

Flow Control: Yes, in combination with Connecting Ring

Mesh Fixation: Injected in housing for strong hold

Fabric material: PET (Polyethylenterephthalat)

Housing Material: LD-PE (Low Density Polyethylen)

Mesh Type: woven

Handling: Extended lip on strainer enables aseptic handling

Sterility: NON-Sterile

Centrifugable: Yes

Tissue Dissociation: Yes

Disinfectable: Yes, with 70% ethanol

Area of interest: Immunology, Cell Biology, Plant Biology, Microbiology, Oncology, Ecology

Shipping Condition: Room Termperature

Storage Condition: Room Temperature

The pluriStrainer® is a sieving device (or cell strainer) for a wide range of laboratory applications where the filtration and the purification of liquids is required. Its unique design features improved ventilation to avoid clogging for a smart sample preparation. pluriStrainer® are stackable to allow direct filtration with different mesh sizes and alsocan be inverted to recover the sieved material. For large sample volumes it can be combined with a funnel. It fits all major brand 50 mL centrifuge (conical) tubes. The combination of the pluriStrainer® and the Connector Ring allow using low pressure which supports the filtration. Furthermore it is possible to block the flow through the sieve and hold back the liquid on top of the mesh. The liquid on top of the mesh can be used for sample preparation and incubation such as tissue dissociation or the treatment of cells with cell lysis reagents (e.g. Buffer RLT, TRIzol®).

• Filter

Attach pluriStrainer® to a sterile 50 mL centrifuge tube. Then, add sample material onto the strainer and filter sample

• Reverse

To obtain the larger fraction, take off the pluriStrainer®, turn it upside down onto another 50 mL tube and flush back the sample from the pluriStrainer®.

• Increase sample load

With Funnel You can add up to 24 mL sample material on top.

• Stack

Stacking of pluriStrainer® with different mesh sizes allows for straining various cell sizes at the same time.

• Flow control

Allows to control the rate of flow by opening or closing the Luer-Lock, e.g. for physical dissociation of primary tissue (brain, spleen etc.).

• Low pressure

If you add a syringe to the Connector Ring, it is possible to force low pressure to support the straining of rough sample material while pulling the piston.

Application

• Obtaining a real single cell suspension after digestion of mammary tissue and organoids
• Enrichment of specific cells using pluriBead technology
• Preparation of a single cell suspension of blood cells from bone marrow, pancreas, thymus, lymph nodes and others
• Dissociation of cells from other primary tissue
• Preparation of real single cell suspension for flow cytometry(FACS™)
• Faster and easier alternative to gauze filtration
• Sieving of complex and viscous liquids (in combination with Connector Ring & Syringe for vacuum)
• Short time incubation of cells / cytokine (in combination with pluriBead and Connector Ring)

Mesh Size: 100 µm

Color: neon yellow

Flow Control: Yes, in combination with Connecting Ring

Mesh Fixation: Injected in housing for strong hold

Fabric material: PET (Polyethylenterephthalat)

Housing Material: LD-PE (Low Density Polyethylen)

Mesh Type: woven

Handling: Extended lip on strainer enables aseptic handling

Sterility: Sterile

Centrifugable: Yes

Tissue Dissociation: Yes

Disinfectable: Yes, with 70% ethanol

Area of interest: Immunology, Cell Biology, Plant Biology, Microbiology, Oncology, Ecology

Shipping Condition: Room Termperature

Storage Condition: Room Temperature