Corning® Epidermal Growth Factor (EGF), human recombinant, 100µg, is a low-molecular-weight mitogenic protein that stimulates proliferation of a wide variety of cell types in vitro. 1/pack. These growth factors have been used to promote long-term proliferation in cell culture and to induce cell differentiation in a variety of cell types under serum-free or reduced serum conditions.
Formulation
Lyophilized from acetic acid, carrier-free; reconstitute in dH2O or aqueous buffer
Preparation and Storage
Stable for at least three months at 2° to 8°C (lyophilized) or = three months at -20°C (solubilized). Do not store in frost-free freezer. Avoid multiple freeze/thaws.
Quality
- Purity: >98% by SDS-PAGE and reverse phase HPLC
- Shown to stimulate proliferation of human foreskin fibroblasts in serum-containing medium
- Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma
Source
Recombinant, E. coli
Molecular Weight
6.2 kD
Tip
Recommended concentration: 0.1 to 1.0 ng/ mL medium depending on cell type